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And So We Meet Again: Allopatric Divergence and Secondary Contact with Gene Flow is a Recurring Them

Perry, Blair W. blair.perry@uta.edu

Schield, Drew R.

Adams, Richard H. Department of Biology

University of Texas at Arlington Arlington, Texas, USA

Card, Daren C.

Department of Biology

University of Texas at Arlington Arlington, Texas, USA

Department of Organismic and Evolutionary Biology Museum of Comparative Zoology

Harvard University

Cambridge, Massachusetts, USA

Jezkova, Tereza

Department of Biology

Miami University of Ohio Oxford, Ohio, USA

Pasquesi, Giulia I. M.

Nikolakis, Zachary L.

Row, Kristopher Department of Biology

University of Texas at Arlington Arlington, Texas, USA

Meik, Jesse M.

Department of Biological Sciences Tarleton State University Stephenville, Texas, USA

Smith, Cara F.

Mackessy, Stephen P.

School of Biological Sciences

University of Northern Colorado

Greeley, Colorado, USA

Castoe, Todd A. Department of Biology

University of Texas at Arlington Arlington, Texas, USA

The study of recently diverged lineages whose geographic ranges come into contact can provide insight into the early stages of speciation and the potential roles of reproductive isolation in generating and maintaining species. Such insight can also be important for understanding the strategies and challenges for delimiting species within recently diverged species complexes. Here, we used mitochondrial and nuclear genetic data to study population structure, gene flow, and demographic history across a geographically widespread rattlesnake clade, the western rattlesnake species complex (Crotalus cerberus, C. viridis, C. oreganus, and relatives), which contains multiple lineages with ranges that geographically overlap or contact one another. We find evidence that pervasive gene flow has broadly influenced patterns of present-day genetic diversity. Our results suggest that lineage diversity has been shaped largely by drift and divergent selection in isolation, followed by secondary contact in which reproductive isolating mechanisms appear weak and insufficient to prevent introgression, even between anciently diverged lineages. The complexity of divergence and secondary contact with gene flow among lineages also provides new context for why delimiting species within this complex has been historically difficult and contentious.


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